The Metabolically Active Cell-associated Matrix of Chondrocytes Is Most Susceptible to the Deleterious Effects of Lipopolysaccarides
نویسنده
چکیده
INTRODUCTION: We recently suggested that chondrocytes that are metabolically most active may make the tissue in which they reside more susceptible to undergo proteoglycan (PG)-depletion following exposure to cytokines and related factors [1]. Using chondrocytes maintained in alginate gel, we also have observed that the metabolically active cell-associated matrix (CM) is more susceptible to interleukin-1 (IL-1)-induced PG depletion than the more voluminous but less metabolically active further removed matrix (FRM) [2]. Articular cartilage exposed to lipopolysaccharides (LPS) molecules present on the cell wall of gram-negative bacteria, undergoes rapid depletion of proteoglycans (PGs), both in vitro [3] and in vivo [4]. LPS is one of the major factors playing a role in the pathogenesis of septic arthritis and lyme arthritis [5]. The purpose of this study was to investigate whether these effects upon the metabolism of chondrocytes also apply when hyperactive chondrocytes are exposed to LPS.
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